Molecular cloning and expression of a new gene, GON-SJTU1 in the rat testis.

ABSTRACT:

Background

Spermatogenesis is a complex process involving cell development, differentiation and apoptosis. This process is governed by a series of genes whose expressions are highly regulated. Male infertility can be attributed to multiple genetic defects or alterations that are related to spermatogenesis. The discovery, cloning and further functional study of genes related to spermatogenesis is of great importance to the elucidation of the molecular mechanism of spermatogenesis. It is also physiologically and pathologically significant to the therapy of male infertility.

Methods

GON-SJTU1 was identified and cloned from rat testis by cDNA library screening and 3'-and 5'-RACE. The products of GON-SJTU1 were assessed by Northern and Western blotting. The expression of GON-SJTU

SUBMITTER: Yang ZJ 

PROVIDER: S-EPMC2887876 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature