Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE.

ABSTRACT: Escherichia coli can survive extreme acid stress for several hours. The most efficient acid resistance system is based on glutamate decarboxylation by the GadA and GadB decarboxylases and the import of glutamate via the GadC membrane protein. The expression of the corresponding genes is controlled by GadE, the central activator of glutamate-dependent acid resistance (GDAR). We have previously shown by genetic approaches that as well as GadE, the response regulator of the Rcs system, RcsB is absolutely required for control of gadA/BC transcription. In the presence of GadE, basal activity of RcsB stimulates the expression of gadA/BC, whereas activation of RcsB leads to general repression of the gad genes. We report here the results of various in vitro assays that show RcsB to regulate by dir

SUBMITTER: Castanie-Cornet MP 

PROVIDER: S-EPMC2887963 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature