Slit3 inhibits Robo3-induced invasion of synovial fibroblasts in rheumatoid arthritis.

ABSTRACT:

Introduction

The repellent factor family of Slit molecules has been described to have repulsive function in the developing nervous system on growing axons expressing the Robo receptors. However, until today no data are available on whether these repellent factors are involved in the regulation of synovial fibroblast (SF) activity in rheumatoid arthritis (RA).

Methods

mRNA expression in primary synovial fibroblasts was quantified by quantitative reverse transcription PCR and protein expression was measured by fluorescence activated cell sorting (FACS) analysis. Different functional assays were performed with rheumatoid arthritis synovial fibroblasts (RASF): proliferation, migration and a novel in-vitro cartilage destruction assay.

Results

First, we found increased expr

SUBMITTER: Denk AE 

PROVIDER: S-EPMC2888193 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature