Calcium deposition in osteoarthritic meniscus and meniscal cell culture.

ABSTRACT:

Introduction

Calcium crystals exist in the knee joint fluid of up to 65% of osteoarthritis (OA) patients and the presence of these calcium crystals correlates with the radiographic evidence of hyaline cartilaginous degeneration. This study sought to examine calcium deposition in OA meniscus and to investigate OA meniscal cell-mediated calcium deposition. The hypothesis was that OA meniscal cells may play a role in pathological meniscal calcification.

Methods

Studies were approved by our human subjects Institutional Review Board. Menisci were collected during joint replacement surgeries for OA patients and during limb amputation surgeries for osteosarcoma patients. Calcium deposits in menisci were examined by alizarin red staining. Expression of genes involved in biomineraliz

SUBMITTER: Sun Y 

PROVIDER: S-EPMC2888206 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature