Gas6-Axl pathway: the role of redox-dependent association of Axl with nonmuscle myosin IIB.

ABSTRACT: In vascular smooth muscle cells, Axl is a key receptor tyrosine kinase, because it is upregulated in injury, increases migration and neointima formation, and is activated by reactive oxygen species. Reaction of glutathione with cysteine residues (termed "glutathiolation") is an important posttranslational redox modification that may alter protein activity and protein-protein interactions. To investigate the mechanisms by which reactive oxygen species increase Axl-dependent vascular smooth muscle cell function we assayed for glutathiolated proteins that associated with Axl in a redox-dependent manner. We identified glutathiolated nonmuscle myosin heavy chain (MHC)-IIB as a novel Axl interacting protein. This interaction was specific in that other myosins did not interact with Axl. The endog

SUBMITTER: Cavet ME 

PROVIDER: S-EPMC2888491 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature