A novel mouse c-fos intronic promoter that responds to CREB and AP-1 is developmentally regulated in vivo.

ABSTRACT:

Background

The c-fos proto-oncogene is an archetype for rapid and integrative transcriptional activation. Innumerable studies have focused on the canonical promoter, located upstream from the transcriptional start site. However, several regulatory sequences have been found in the first intron.

Methodology/principal findings

Here we describe an extremely conserved region in c-fos first intron that contains a putative TATA box, and functional TRE and CRE sites. This fragment drives reporter gene activation in fibroblasts, which is enhanced by increasing intracellular calcium and cAMP and by cotransfection of CREB or c-Fos/c-Jun expression vectors. We produced transgenic mice expressing a lacZ reporter controlled by the intronic promoter. Lac Z expression of this promoter is re

SUBMITTER: Coulon V 

PROVIDER: S-EPMC2888593 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature