Zebrafish fetal alcohol syndrome model: effects of ethanol are rescued by retinoic acid supplement.

ABSTRACT: This study was designed to develop a zebrafish experimental model to examine defects in retinoic acid (RA) signaling caused by embryonic ethanol exposure. RA deficiency may be a causative factor leading to a spectrum of birth defects classified as fetal alcohol spectrum disorder (FASD). Experimental support for this hypothesis using Xenopus showed that effects of treatment with ethanol could be partially rescued by adding retinoids during ethanol treatment. Previous studies show that treating zebrafish embryos during gastrulation and somitogenesis stages with a pathophysiological concentration of ethanol (100mM) produces effects that are characteristic features of FASD. We found that treating zebrafish embryos with RA at a low concentration (10(-9)M) and 100mM ethanol during gastrulation a

SUBMITTER: Marrs JA 

PROVIDER: S-EPMC2889201 | biostudies-literature | 2010 Nov-Dec

REPOSITORIES: biostudies-literature