Regulation of MafA expression in pancreatic beta-cells in db/db mice with diabetes.

ABSTRACT:

Objective

Islet beta-cells loose their ability to synthesize insulin under diabetic conditions, which is at least partially due to the decreased activity of insulin transcription factors such as MafA. Although an in vitro study showed that reactive oxygen species (ROS) decrease MafA expression, the underlying mechanism still remains unclear. In this study, we examined the effects of c-Jun, which is known to be upregulated by ROS, on the expression of MafA under diabetic conditions.

Research design and methods

To examine the protein levels of MafA and c-Jun, we performed histological analysis and Western blotting using diabetic db/db mice. In addition, to evaluate the possible effects of c-Jun on MafA expression, we performed adenoviral overexpression of c-Jun in the MIN6 bet

SUBMITTER: Matsuoka TA 

PROVIDER: S-EPMC2889771 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature