Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin.

ABSTRACT:

Background

Chondroitin sulphate is a complex polysaccharide having important structural and protective functions in animal tissues. Extracted from animals, this compound is used as a human anti-inflammatory drug. Among bacteria, Escherichia coli K4 produces a capsule containing a non-sulphate chondroitin and its development may provide an efficient and cheap fermentative production of the polysaccharide.

Results

A random N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis was performed on E. coli K4 to isolate mutants showing an increased production of chondroitin. Several mutants were isolated, one of which, here named VZ15, produced about 80% more chondroitin than the wild type E. coli. We found that the mutant has a missense mutation in the codon 313 of kfoC, the gene encodi

SUBMITTER: Zanfardino A 

PROVIDER: S-EPMC2889854 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature