High-throughput miRNA profiling of human melanoma blood samples.

ABSTRACT:

Background

MicroRNA (miRNA) signatures are not only found in cancer tissue but also in blood of cancer patients. Specifically, miRNA detection in blood offers the prospect of a non-invasive analysis tool.

Methods

Using a microarray based approach we screened almost 900 human miRNAs to detect miRNAs that are deregulated in their expression in blood cells of melanoma patients. We analyzed 55 blood samples, including 20 samples of healthy individuals, 24 samples of melanoma patients as test set, and 11 samples of melanoma patients as independent validation set.

Results

A hypothesis test based approach detected 51 differentially regulated miRNAs, including 21 miRNAs that were downregulated in blood cells of melanoma patients and 30 miRNAs that were upregulated in blood ce

SUBMITTER: Leidinger P 

PROVIDER: S-EPMC2889897 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature