Selection of optimal reference genes for normalization in quantitative RT-PCR.

ABSTRACT:

Background

Normalization in real-time qRT-PCR is necessary to compensate for experimental variation. A popular normalization strategy employs reference gene(s), which may introduce additional variability into normalized expression levels due to innate variation (between tissues, individuals, etc). To minimize this innate variability, multiple reference genes are used. Current methods of selecting reference genes make an assumption of independence in their innate variation. This assumption is not always justified, which may lead to selecting a suboptimal set of reference genes.

Results

We propose a robust approach for selecting optimal subset(s) of reference genes with the smallest variance of the corresponding normalizing factors. The normalizing factor variance estimates ar

SUBMITTER: Chervoneva I 

PROVIDER: S-EPMC2889935 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature