Development of genome-specific primers for homoeologous genes in allopolyploid species: the waxy and starch synthase II genes in allohexaploid wheat (Triticum aestivum L.) as examples.

ABSTRACT:

Background

In allopolypoid crops, homoeologous genes in different genomes exhibit a very high sequence similarity, especially in the coding regions of genes. This makes it difficult to design genome-specific primers to amplify individual genes from different genomes. Development of genome-specific primers for agronomically important genes in allopolypoid crops is very important and useful not only for the study of sequence diversity and association mapping of genes in natural populations, but also for the development of gene-based functional markers for marker-assisted breeding. Here we report on a useful approach for the development of genome-specific primers in allohexaploid wheat.

Findings

In the present study, three genome-specific primer sets for the waxy (Wx) genes and

SUBMITTER: Huang XQ 

PROVIDER: S-EPMC2890506 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature