Dependency on de novo protein synthesis and proteomic changes during metamorphosis of the marine bryozoan Bugula neritina.

ABSTRACT:

Background

Metamorphosis in the bryozoan Bugula neritina (Linne) includes an initial phase of rapid morphological rearrangement followed by a gradual phase of morphogenesis. We hypothesized that the first phase may be independent of de novo synthesis of proteins and, instead, involves post-translational modifications of existing proteins, providing a simple mechanism to quickly initiate metamorphosis. To test our hypothesis, we challenged B. neritina larvae with transcription and translation inhibitors. Furthermore, we employed 2D gel electrophoresis to characterize changes in the phosphoproteome and proteome during early metamorphosis. Differentially expressed proteins were identified by liquid chromatography tandem mass spectrometry and their gene expression patterns were profile

SUBMITTER: Wong YH 

PROVIDER: S-EPMC2890537 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature