Proteomic analysis of the fish pathogen Flavobacterium columnare.

ABSTRACT:

Background

Flavobacterium columnare causes columnaris disease in cultured and wild fish populations worldwide. Columnaris is the second most prevalent bacterial disease of commercial channel catfish industry in the United States. Despite its economic importance, little is known about the expressed proteins and virulence mechanisms of F. columnare. Here, we report the first high throughput proteomic analysis of F. columnare using 2-D LC ESI MS/MS and 2-DE MALDI TOF/TOF MS.

Results

Proteins identified in this study and predicted from the draft F. columnare genome were clustered into functional groups using clusters of orthologous groups (COGs), and their subcellular locations were predicted. Possible functional relations among the identified proteins were determined using path

SUBMITTER: Dumpala PR 

PROVIDER: S-EPMC2890538 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature