Two-photon imaging of the trabecular meshwork.

ABSTRACT:

Purpose

To image the trabecular meshwork (TM) in its native unfixed state using a non-invasive, non-destructive technique.

Methods

Two-photon microscopy (2PM), including two-photon excitation fluorescence (2PEF) and second harmonic generation (SHG), was used to image flat-mounted trabecular meshwork samples from human cadaver eyes. Multiple images were analyzed along the tissue axis (z-axis) to generate a three-dimensional (3D) model of the region.

Results

A lattice of large collagen fibers (approximately 10 microm in diameter) were detected by inherent fluorescence (2PEF) and SHG. There are regions of both tightly overlapping bundles as well as fluid-filled regions visible from the surface of the TM. 3D analysis of multiple images reveals that the open regions deep i

SUBMITTER: Ammar DA 

PROVIDER: S-EPMC2890557 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature