Characterisation of sugar beet (Beta vulgaris L. ssp. vulgaris) varieties using microsatellite markers.

ABSTRACT:

Background

Sugar beet is an obligate outcrossing species. Varieties consist of mixtures of plants from various parental combinations. As the number of informative morphological characteristics is limited, this leads to some problems in variety registration research.

Results

We have developed 25 new microsatellite markers for sugar beet. A selection of 12 markers with high quality patterns was used to characterise 40 diploid and triploid varieties. For each variety 30 individual plants were genotyped. The markers amplified 3-21 different alleles. Varieties had up to 7 different alleles at one marker locus. All varieties could be distinguished. For the diploid varieties, the expected heterozygosity ranged from 0.458 to 0.744. The average inbreeding coefficient F(is) was 0.282

SUBMITTER: Smulders MJ 

PROVIDER: S-EPMC2890681 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature