Parent-independent genotyping for constructing an ultrahigh-density linkage map based on population sequencing.

ABSTRACT: Bar-coded multiplexed sequencing approaches based on new-generation sequencing technologies provide capacity to sequence a mapping population in a single sequencing run. However, such approaches usually generate low-coverage and error-prone sequences for each line in a population. Thus, it is a significant challenge to genotype individual lines in a population for linkage map construction based on low-coverage sequences without the availability of high-quality genotype data of the parental lines. In this paper, we report a method for constructing ultrahigh-density linkage maps composed of high-quality single-nucleotide polymorphisms (SNPs) based on low-coverage sequences of recombinant inbred lines. First, all potential SNPs were identified to obtain drafts of parental genotypes using a ma

SUBMITTER: Xie W 

PROVIDER: S-EPMC2890813 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature