Duct system of the rabbit lacrimal gland: structural characteristics and role in lacrimal secretion.

ABSTRACT:

Purpose

To develop a nomenclature for the lacrimal duct system in the rabbit, based on the anatomic and structural characteristics of each duct segment, and to provide RT-PCR and immunofluorescence data to support the notion that the duct system plays important roles in lacrimal function.

Methods

Paraffin-embedded lacrimal glands (LGs) were stained with hematoxylin and eosin (H&E) and evaluated with a stereomicroscope. Cryosections of LG were stained with cresyl violet, and acinar cells and ductal epithelial cells were isolated from each duct segment by laser capture microdissection (LCM). mRNA levels from these cells were analyzed by real-time RT-PCR. Standard protocol was followed for immunofluorescence detection of ionic transporters.

Results

The lacrimal duct syst

SUBMITTER: Ding C 

PROVIDER: S-EPMC2891459 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature