MicroRNA-29a regulates intestinal membrane permeability in patients with irritable bowel syndrome.

ABSTRACT:

Background

The molecular mechanisms underlying the pathophysiology of irritable bowel syndrome (IBS) are poorly understood. One mechanism may involve increased intestinal permeability that is reversed with glutamine supplementation. Our goal was to evaluate the expression of glutamine synthetase and its complementary miRNA in blood microvesicles and gut tissues of IBS patients with increased intestinal membrane permeability.

Methods

We evaluated 19 diarrhoea-predominant IBS patients and 10 controls for intestinal membrane permeability using the lactulose/mannitol method. miRNA expression was evaluated in blood microvesicles and gut tissue. To further confirm the relationship between miRNA and glutamine synthetase expression, cell culture experiments were conducted. Glutamine

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC2891786 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature