A short upstream promoter region mediates transcriptional regulation of the mouse doublecortin gene in differentiating neurons.

ABSTRACT:

Background

Doublecortin (Dcx), a MAP (Microtubule-Associated Protein), is transiently expressed in migrating and differentiating neurons and thereby characterizes neuronal precursors and neurogenesis in developing and adult neurogenesis. In addition, reduced Dcx expression during development has been related to appearance of brain pathologies. Here, we attempt to unveil the molecular mechanisms controlling Dcx gene expression by studying its transcriptional regulation during neuronal differentiation.

Results

To determine and analyze important regulatory sequences of the Dcx promoter, we studied a putative regulatory region upstream from the mouse Dcx coding region (pdcx2kb) and several deletions thereof. These different fragments were used in vitro and in vivo to drive repor

SUBMITTER: Piens M 

PROVIDER: S-EPMC2891791 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature