Chromatin and sequence features that define the fine and gross structure of genomic methylation patterns.

ABSTRACT: Abnormalities of genomic methylation patterns are lethal or cause disease, but the cues that normally designate CpG dinucleotides for methylation are poorly understood. We have developed a new method of methylation profiling that has single-CpG resolution and can address the methylation status of repeated sequences. We have used this method to determine the methylation status of >275 million CpG sites in human and mouse DNA from breast and brain tissues. Methylation density at most sequences was found to increase linearly with CpG density and to fall sharply at very high CpG densities, but transposons remained densely methylated even at higher CpG densities. The presence of histone H2A.Z and histone H3 di- or trimethylated at lysine 4 correlated strongly with unmethylated DNA and occurred

SUBMITTER: Edwards JR 

PROVIDER: S-EPMC2892098 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature