A new approach to characterize populations of Schistosoma mansoni from humans: development and assessment of microsatellite analysis of pooled miracidia.

ABSTRACT:

Objectives

To develop and assess a microsatellite technique to characterize populations of Schistosoma mansoni from humans.

Methods

For each of five patients, we calculated the allele count and frequency at 11 loci for several pools of miracidia (50 and 100), and compared these to population values, determined by amplifying microsatellites from 186 to 200 individual miracidia per patient.

Results

We were able to detect up to 94.5% of alleles in pools. Allele count and frequency strongly and significantly correlated between singles and pools; marginally significant differences (P < 0.05) were detected for one patient (pools of 50) for allele frequencies and for two patients (pools of 100) for allele counts. Kato-Katz egg counts and number of alleles per pool did not co

SUBMITTER: Hanelt B 

PROVIDER: S-EPMC2892310 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature