An induced mutation in tomato eIF4E leads to immunity to two potyviruses.

ABSTRACT:

Background

The characterization of natural recessive resistance genes and Arabidopsis virus-resistant mutants have implicated translation initiation factors of the eIF4E and eIF4G families as susceptibility factors required for virus infection and resistance function.

Methodology/principal findings

To investigate further the role of translation initiation factors in virus resistance we set up a TILLING platform in tomato, cloned genes encoding for translation initiation factors eIF4E and eIF4G and screened for induced mutations that lead to virus resistance. A splicing mutant of the eukaryotic translation initiation factor, S.l_eIF4E1 G1485A, was identified and characterized with respect to cap binding activity and resistance spectrum. Molecular analysis of the transcript of

SUBMITTER: Piron F 

PROVIDER: S-EPMC2892489 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature