An evaluation of the PCR-RFLP technique to aid molecular-based monitoring of felids and canids in India.

ABSTRACT:

Background

The order Carnivora is well represented in India, with 58 of the 250 species found globally, occurring here. However, small carnivores figure very poorly in research and conservation policies in India. This is mainly due to the dearth of tested and standardized techniques that are both cost effective and conducive to small carnivore studies in the field. In this paper we present a non-invasive genetic technique standardized for the study of Indian felids and canids with the use of PCR amplification and restriction enzyme digestion of scat collected in the field.

Findings

Using existing sequences of felids and canids from GenBank, we designed primers from the 16S rRNA region of the mitochondrial genome and tested these on ten species of felids and five canids. We s

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC2892506 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature