Differential regulation of vascular tone and remodeling via stimulation of type 2 and type 6 adenylyl cyclases in the ductus arteriosus.

ABSTRACT:

Rationale

Prostaglandin (PG)E(2), which increases intracellular cAMP via activation of adenylyl cyclases (ACs), induces vasodilation and hyaluronan-mediated intimal thickening (IT) in the ductus arteriosus (DA) during late gestation. After birth, however, differential regulation of vasodilation and IT is preferable for treatment of patients with patent DA and DA-dependent congenital cardiac malformations.

Objective

Our objectives were to examine whether AC isoforms play differential roles in DA vasodilation and IT.

Methods and results

AC2 and AC6 were more highly expressed in rat DA than in the aorta during the perinatal period. AC6-targeted siRNA counteracted PGE(1)-induced hyaluronan production in rat DA smooth muscle cells. Overexpression of AC6 enhanced PGE(1)-ind

SUBMITTER: Yokoyama U 

PROVIDER: S-EPMC2892563 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature