Characterization of the mouse CP27 promoter and NF-Y mediated gene regulation.

ABSTRACT: The cp27 gene is a highly conserved and unique gene with important roles related to craniofacial organogenesis. The present study is a first analysis of the CP27 promoter and its regulation. Here, we have cloned the promoter of the mouse cp27 gene, examined its transcriptional activity, and identified transcription factor binding sites in the proximal promoter region. Two major transcription start sites were mapped adjacent to exon 1. Promoter function analysis of the 5' flanking region by progressive 5' deletion mutations localized transcription repression elements between -1993bp and -969bp and several positive elements between -968bp and the preferred transcription start site. EMSA and functional studies indicated two function-cooperative CCAAT boxes and identified the NF-Y transcriptio

SUBMITTER: Luan X 

PROVIDER: S-EPMC2892892 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature