Centriole movements in mammalian epithelial cells during cytokinesis.

ABSTRACT:

Background

In cytokinesis, when the cleavage furrow has been formed, the two centrioles in each daughter cell separate. It has been suggested that the centrioles facilitate and regulate cytokinesis to some extent. It has been postulated that termination of cytokinesis (abscission) depends on the migration of a centriole to the intercellular bridge and then back to the cell center. To investigate the involvement of centrioles in cytokinesis, we monitored the movements of centrioles in three mammalian epithelial cell lines, HeLa, MCF 10A, and the p53-deficient mouse mammary tumor cell line KP-7.7, by time-lapse imaging. Centrin1-EGFP and alpha-Tubulin-mCherry were co-expressed in the cells to visualize respectively the centrioles and microtubules.

Results

Here we report that s

SUBMITTER: Jonsdottir AB 

PROVIDER: S-EPMC2893098 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature