Mycobacterium bovis-BCG vaccination induces specific pulmonary transcriptome biosignatures in mice.

ABSTRACT:

Background

In the present study, we applied microarray technology to define biosignatures by microarray transcriptome analysis in lung and spleen samples after BCG vaccination and M. bovis infection of BALB/c mice. The aims were two-fold, namely to define biosignatures that could predict vaccine success before challenge, and biomarker patterns that correlated with anamnestic protective responses following exposure to virulent M. bovis. Further, these biosignatures should be detectable without in vitro antigenic challenge.

Principal findings

After BCG vaccination, we defined a specific pulmonary gene expression signature related to the connective tissue development and function network that predicted vaccine success before M. bovis challenge. In addition, a Th17-related cytok

SUBMITTER: Aranday Cortes E 

PROVIDER: S-EPMC2893133 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature