Development and validation of a method for profiling post-translational modification activities using protein microarrays.

ABSTRACT:

Background

Post-translational modifications (PTMs) impact on the stability, cellular location, and function of a protein thereby achieving a greater functional diversity of the proteome. To fully appreciate how PTMs modulate signaling networks, proteome-wide studies are necessary. However, the evaluation of PTMs on a proteome-wide scale has proven to be technically difficult. To facilitate these analyses we have developed a protein microarray-based assay that is capable of profiling PTM activities in complex biological mixtures such as whole-cell extracts and pathological specimens.

Methodology/principal findings

In our assay, protein microarrays serve as a substrate platform for in vitro enzymatic reactions in which a recombinant ligase, or extracts prepared from whole cell

SUBMITTER: Del Rincon SV 

PROVIDER: S-EPMC2893156 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature