Characterization of strains unlike Mesorhizobium loti that nodulate lotus spp. in saline soils of Granada, Spain.

ABSTRACT: Lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. The aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with Lotus spp. A total of 180 isolates able to nodulate Lotus corniculatus and Lotus tenuis from two locations in Granada, Spain, were characterized. Molecular identification of the isolates was performed by repetitive extragenic palindromic PCR (REP-PCR) and 16S rRNA, atpD, and recA gene sequence analyses, showing the presence of bacteria related to different species of the genus Mesorhizobium: Mesorhizobium tarimense/Mesorhizobium tianshanense, Mesorhizobium chacoens

SUBMITTER: Lorite MJ 

PROVIDER: S-EPMC2893469 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature