Comparison of normalization methods for construction of large, multiplex amplicon pools for next-generation sequencing.

ABSTRACT: Constructing mixtures of tagged or bar-coded DNAs for sequencing is an important requirement for the efficient use of next-generation sequencers in applications where limited sequence data are required per sample. There are many applications in which next-generation sequencing can be used effectively to sequence large mixed samples; an example is the characterization of microbial communities where

SUBMITTER: Harris JK 

PROVIDER: S-EPMC2893486 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature