In planta gene expression analysis of Xanthomonas oryzae pathovar oryzae, African strain MAI1.

ABSTRACT:

Background

Bacterial leaf blight causes significant yield losses in rice crops throughout Asia and Africa. Although both the Asian and African strains of the pathogen, Xanthomonas oryzae pv. oryzae (Xoo), induce similar symptoms, they are nevertheless genetically different, with the African strains being more closely related to the Asian X. oryzae pv. oryzicola (Xoc).

Results

Changes in gene expression of the African Xoo strain MAI1 in the susceptible rice cultivar Nipponbare were profiled, using an SSH Xoo DNA microarray. Microarray hybridization was performed comparing bacteria recovered from plant tissues at 1, 3, and 6 days after inoculation (dai) with bacteria grown in vitro. A total of 710 bacterial genes were found to be differentially expressed, with 407 up-regulated

SUBMITTER: Soto-Suarez M 

PROVIDER: S-EPMC2893596 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature