Combining next-generation sequencing strategies for rapid molecular resource development from an invasive aphid species, Aphis glycines.

ABSTRACT:

Background

Aphids are one of the most important insect taxa in terms of ecology, evolutionary biology, genetics and genomics, and interactions with endosymbionts. Additionally, many aphids are serious pest species of agricultural and horticultural plants. Recent genetic and genomic research has expanded molecular resources for many aphid species, including the whole genome sequencing of the pea aphid, Acrythosiphon pisum. However, the invasive soybean aphid, Aphis glycines, lacks in any significant molecular resources.

Methodology/principal findings

Two next-generation sequencing technologies (Roche-454 and Illumina GA-II) were used in a combined approach to develop both transcriptomic and genomic resources, including expressed genes and molecular markers. Over 278 million b

SUBMITTER: Bai X 

PROVIDER: S-EPMC2894077 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature