Altered neuronatin expression in the rat dorsal root ganglion after sciatic nerve transection.

ABSTRACT:

Background

Several molecular changes occur following axotomy, such as gene up-regulation and down-regulation. In our previous study using Affymetrix arrays, it was found that after the axotomy of sciatic nerve, there were many novel genes with significant expression changes. Among them, neuronatin (Nnat) was the one which expression was significantly up-regulated. Nnat was identified as a gene selectively expressed in neonatal brains and markedly reduced in adult brains. The present study investigated whether the expression of Nnat correlates with symptoms of neuropathic pain in adult rats with transected sciatic nerve.

Methods

Western blotting, immunohistochemistry, and the Randall and Selitto test were used to study the protein content, and subcellular localization of Nnat

SUBMITTER: Chen KH 

PROVIDER: S-EPMC2894761 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature