Identification of inappropriately reprogrammed genes by large-scale transcriptome analysis of individual cloned mouse blastocysts.

ABSTRACT: Although cloned embryos generated by somatic/embryonic stem cell nuclear transfer (SECNT) certainly give rise to viable individuals, they can often undergo embryonic arrest at any stage of embryogenesis, leading to diverse morphological abnormalities. In an effort to gain further insights into reprogramming and the properties of SECNT embryos, we performed a large-scale gene expression profiling of 87 single blastocysts using GeneChip microarrays. Sertoli cells, cumulus cells, and embryonic stem cells were used as donor cells. The gene expression profiles of 87 blastocysts were subjected to microarray analysis. Using principal component analysis and hierarchical clustering, the gene expression profiles were clearly classified into 3 clusters corresponding to the type of donor cell. The res

SUBMITTER: Fukuda A 

PROVIDER: S-EPMC2894852 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature