Endothelial [Ca2+]i and caveolin-1 antagonistically regulate eNOS activity and microvessel permeability in rat venules.

ABSTRACT:

Aims

In this study, we investigated the mechanisms by which caveolin-1 (CAV) inhibits increases in permeability induced by platelet activating factor (PAF) and elucidated the relationship between the endothelial intracellular Ca(2+) concentration ([Ca(2+)](i)) and CAV in regulating endothelial nitric oxide synthase (eNOS) activity and microvessel permeability in intact microvessels.

Methods and results

Experiments were conducted in individually perfused mesenteric venules in Sprague-Dawley rats. Permeability was determined by measuring hydraulic conductivity (Lp). Endothelial [Ca(2+)](i) and nitric oxide (NO) production were measured in fura-2- and DAF-2-loaded microvessels. Perfusion of the CAV scaffolding domain, AP-CAV, at 1 microM for 30 min did not affect PAF-induced in

SUBMITTER: Zhou X 

PROVIDER: S-EPMC2895537 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature