Interleukin-1beta promotes gastric atrophy through suppression of Sonic Hedgehog.

ABSTRACT:

Background & aims

In both human subjects and rodent models, Helicobacter infection leads to a decrease in Shh expression in the stomach. Sonic Hedgehog (Shh) is highly expressed in the gastric corpus and its loss correlates with gastric atrophy. Therefore, we tested the hypothesis that proinflammatory cytokines induce gastric atrophy by inhibiting Shh expression.

Methods

Shh-LacZ reporter mice were infected with Helicobacter felis for 3 and 8 weeks. Changes in Shh expression were monitored using beta-galactosidase staining and immunohistochemistry. Gastric acidity was measured after infection, and interleukin (IL)-1beta was quantified by quantitative reverse-transcription polymerase chain reaction. Mice were injected with either IL-1beta or omeprazole before measuring Shh mR

SUBMITTER: Waghray M 

PROVIDER: S-EPMC2895809 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature