Observing cycling of a few cross-bridges during isometric contraction of skeletal muscle.

ABSTRACT: During muscle contraction a myosin cross-bridge imparts periodic force impulses to actin. It is possible to visualize those impulses by observing a few molecules of actin or myosin. We have followed the time course of orientation change of a few actin molecules during isometric contraction by measuring parallel polarized intensity of its fluorescence. The orientation of actin reflects local bending of a thin filament and is different when a cross-bridge binds to, or is detached from, F-actin. The changes in orientation were characterized by periods of activity during which myosin cross-bridges interacted normally with actin, interspersed with periods of inactivity during which actin and myosin were unable to interact. The periods of activity lasted on average 1.2 +/- 0.4 s and were separat

SUBMITTER: Mettikolla P 

PROVIDER: S-EPMC2896876 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature