Regulation of high-affinity iron acquisition homologues in the tsetse fly symbiont Sodalis glossinidius.

ABSTRACT: Sodalis glossinidius is a facultative intracellular bacterium that is a secondary symbiont of the tsetse fly (Diptera: Glossinidae). Since studies with other facultative intracellular bacteria have shown that high-affinity iron acquisition genes are upregulated in vivo, we investigated the regulation of several Sodalis genes that encode putative iron acquisition systems. These genes, SG1538 (hemT) and SG1516 (sitA), are homologous to genes encoding periplasmic heme and iron/manganese transporters, respectively. hemT promoter- and sitA promoter-gfp fusions were constructed, and in both Escherichia coli and Sodalis backgrounds, expression levels of these fusions were higher when the bacteria were grown in iron-limiting media than when the bacteria were grown in iron-replete media. The Sodali

SUBMITTER: Runyen-Janecky LJ 

PROVIDER: S-EPMC2897345 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature