Random mutagenesis of Clostridium cellulolyticum by using a Tn1545 derivative.

ABSTRACT: Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.

SUBMITTER: Blouzard JC 

PROVIDER: S-EPMC2897430 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature