Shotgun proteomics of Aspergillus niger microsomes upon D-xylose induction.

ABSTRACT: Protein secretion plays an eminent role in cell maintenance and adaptation to the extracellular environment of microorganisms. Although protein secretion is an extremely efficient process in filamentous fungi, the mechanisms underlying protein secretion have remained largely uncharacterized in these organisms. In this study, we analyzed the effects of the d-xylose induction of cellulase and hemicellulase enzyme secretion on the protein composition of secretory organelles in Aspergillus niger. We aimed to systematically identify the components involved in the secretion of these enzymes via mass spectrometry of enriched subcellular microsomal fractions. Under each condition, fractions enriched for secretory organelles were processed for tandem mass spectrometry, resulting in the identificati

SUBMITTER: Ferreira de Oliveira JM 

PROVIDER: S-EPMC2897441 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature