Unknown

Dataset Information

0

Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant.

ABSTRACT: The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols. Pyramidal crystals grown in the presence of (R)-phenylethanol via the hanging-drop vapour-diffusion method diffracted to 3.2 A resolution at the Canadian Light Source. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.23, b = 124.90, c = 164.80 A. The structure was solved by molecular replacement using the structure of T. brockii SADH (PDB entry 1ykf).

SUBMITTER: Protsko C 

PROVIDER: S-EPMC2898473 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant.

Protsko Carla C   Vieille Claire C   Laivenieks Maris M   Prasad Lata L   Sanders David A R DA   Delbaere Louis T J LT  

Acta crystallographica. Section F, Structural biology and crystallization communications 20100624 Pt 7

The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols. Pyramidal crystals grown in the presence of (R)-phenylethanol via the hanging-drop vapour-diffusion method diffracted to 3.2 A resolution at the Canadian Light Source. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.23, b = 124.90, c = 164.80 A. The structure was solved by molecular replacement using the st  ...[more]

Similar Datasets

Unknown Biophysical and mutagenic analysis of Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase activity and specificity.
| S-EPMC1218725 | biostudies-other
Unknown Crystallization and preliminary X-ray diffraction studies of Staphylococcus aureus homoserine dehydrogenase.
| S-EPMC3818036 | biostudies-literature
Unknown Crystallization and preliminary X-ray diffraction of malate dehydrogenase from Plasmodium falciparum.
| S-EPMC3370904 | biostudies-literature
Unknown Crystallization and preliminary X-ray diffraction analysis of Leishmania major dihydroorotate dehydrogenase.
| S-EPMC2225193 | biostudies-literature
Unknown Crystallization, preliminary X-ray diffraction and structure analysis of Thermotoga maritima mannitol dehydrogenase.
| S-EPMC2330225 | biostudies-literature
Unknown Crystallization and preliminary X-ray diffraction analysis of domain-chimeric L-(2S,3S)-butanediol dehydrogenase.
| S-EPMC3976062 | biostudies-literature
Unknown Cloning and expression of the gene encoding the Thermoanaerobacter ethanolicus 39E secondary-alcohol dehydrogenase and biochemical characterization of the enzyme.
| S-EPMC1217309 | biostudies-other
Unknown Purification of acetaldehyde dehydrogenase and alcohol dehydrogenases from Thermoanaerobacter ethanolicus 39E and characterization of the secondary-alcohol dehydrogenase (2 degrees Adh) as a bifunctional alcohol dehydrogenase--acetyl-CoA reductive thioesterase.
| S-EPMC1137204 | biostudies-other
Unknown Crystallization and X-ray diffraction studies of inverting trehalose phosphorylase from Thermoanaerobacter sp.
| S-EPMC2852340 | biostudies-literature
Unknown Crystallization and preliminary X-ray diffraction analysis of D53H mutant Escherichia coli cAMP receptor protein.
| S-EPMC3855738 | biostudies-literature