Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation.

ABSTRACT: Selective protein cleavage at methionine residues is a useful method for the production of bacterially derived protein fragments containing an N-terminal cysteine residue required for native chemical ligation. Here we describe an optimised procedure for cyanogen bromide-mediated protein cleavage, and ligation of the resulting fragments to afford biologically active proteins.

SUBMITTER: Richardson JP 

PROVIDER: S-EPMC2898651 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature