A rapid and scalable method for selecting recombinant mouse monoclonal antibodies.

ABSTRACT:

Background

Monoclonal antibodies with high affinity and selectivity that work on wholemount fixed tissues are valuable reagents to the cell and developmental biologist, and yet isolating them remains a long and unpredictable process. Here we report a rapid and scalable method to select and express recombinant mouse monoclonal antibodies that are essentially equivalent to those secreted by parental IgG-isotype hybridomas.

Results

Increased throughput was achieved by immunizing mice with pools of antigens and cloning - from small numbers of hybridoma cells - the functionally rearranged light and heavy chains into a single expression plasmid. By immunizing with the ectodomains of zebrafish cell surface receptor proteins expressed in mammalian cells and screening for formalin-re

SUBMITTER: Crosnier C 

PROVIDER: S-EPMC2898661 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature