The methylated N-terminal tail of RCC1 is required for stabilisation of its interaction with chromatin by Ran in live cells.

ABSTRACT:

Background

Regulator of chromosome condensation 1 (RCC1) is the guanine nucleotide exchange factor for Ran GTPase. Localised generation of Ran-GTP by RCC1 on chromatin is critical for nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. Both the N-terminal tail of RCC1 and its association with Ran are important for its interaction with chromatin in cells. In vitro, the association of Ran with RCC1 induces a conformational change in the N-terminal tail that promotes its interaction with DNA.

Results

We have investigated the mechanism of the dynamic interaction of the alpha isoform of human RCC1 (RCC1alpha) with chromatin in live cells using fluorescence recovery after photobleaching (FRAP) of green fluorescent protein (GFP) fusions. We show th

SUBMITTER: Hitakomate E 

PROVIDER: S-EPMC2898669 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature