De novo characterization of a whitefly transcriptome and analysis of its gene expression during development.

ABSTRACT:

Background

Whitefly (Bemisia tabaci) causes extensive crop damage throughout the world by feeding directly on plants and by vectoring hundreds of species of begomoviruses. Yet little is understood about its genes involved in development, insecticide resistance, host range plasticity and virus transmission.

Results

To facilitate research on whitefly, we present a method for de novo assembly of whitefly transcriptome using short read sequencing technology (Illumina). In a single run, we produced more than 43 million sequencing reads. These reads were assembled into 168,900 unique sequences (mean size = 266 bp) which represent more than 10-fold of all the whitefly sequences deposited in the GenBank (as of March 2010). Based on similarity search with known proteins, these analys

SUBMITTER: Wang XW 

PROVIDER: S-EPMC2898760 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature