Molecular analysis of plasma DNA for the early detection of lung cancer by quantitative methylation-specific PCR.

ABSTRACT:

Purpose

Aberrant promoter hypermethylation of tumor suppressor genes is a promising marker for lung cancer detection. We investigated the likelihood of detecting aberrant DNA methylation of tumor suppressor genes in plasma samples of patients with abnormalities of the lung detected upon computed tomography (CT) scan.

Experimental design

In a small evaluation cohort, four gene promoters (DCC, Kif1a, NISCH, and Rarb) were found to be methylated with increased frequency in samples from cancer patients specifically. We then examined DNA from 93 plasma samples from patients with abnormal findings in the lung detected upon CT scan for aberrant methylation of these four gene promoters by quantitative fluorogenic real-time PCR. The patients were divided into two groups, ground glass

SUBMITTER: Ostrow KL 

PROVIDER: S-EPMC2899894 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature