Near-perfect infectivity of wild-type AAV as benchmark for infectivity of recombinant AAV vectors.

ABSTRACT: Viral vectors derived from adeno-associated viruses (AAVs) are widely used for gene transfer both in vitro and in vivo. The increasing use of AAV as a gene transfer vector, as well as recently shown immunological complications in clinical trials, highlight the necessity to define the specific activity of vector preparations beyond current standards. In this report, we determined the infectious, physical and genome-containing particle titers of several wild-type AAV type 2 (wtAAV2) and recombinant AAV type 2 (rAAV2) preparations that were produced and purified by standard methods. We found that the infectivity of wtAAV2 approaches a physical-to-infectious particle ratio of one. This near-perfect physical-to-infectious particle ratio defines a 'ceiling' for the theoretically achievable quali

SUBMITTER: Zeltner N 

PROVIDER: S-EPMC2900506 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature