Controllable Soluble Protein Concentration Gradients in Hydrogel Networks.

ABSTRACT: Here we report controlled formation of sustained, soluble protein concentration gradients within hydrated polymer networks. The approach involves spatially localizing proteins or biodegradable, protein-loaded microspheres within hydrogels to form a protein-releasing "depot". Soluble protein concentration gradients are then formed as the released protein diffuses away from the localized source. Control over key gradient parameters, including maximum concentration, gradient magnitude, slope, and time dynamics, is achieved by controlling the release of protein from the depot and subsequent transport through the hydrogel. Results demonstrate a direct relationship between the amount of protein released from the depot and the source concentration, gradient magnitude, and slope of the concentrati

SUBMITTER: Peret BJ 

PROVIDER: S-EPMC2900850 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature