The Drosophila Anion Exchanger (DAE) lacks a detectable interaction with the spectrin cytoskeleton.

ABSTRACT:

Background

Current models suggest that the spectrin cytoskeleton stabilizes interacting ion transport proteins at the plasma membrane. The human erythrocyte anion exchanger (AE1) was the first membrane transport protein found to be associated with the spectrin cytoskeleton. Here we evaluated a conserved anion exchanger from Drosophila (DAE) as a marker for studies of the downstream effects of spectrin cytoskeleton mutations.

Results

Sequence comparisons established that DAE belongs to the SLC4A1-3 subfamily of anion exchangers that includes human AE1. Striking sequence conservation was observed in the C-terminal membrane transport domain and parts of the N-terminal cytoplasmic domain, but not in the proposed ankyrin-binding site. Using an antibody raised against DAE and a re

SUBMITTER: Dubreuil RR 

PROVIDER: S-EPMC2901199 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature